Colocalization pattern of cocaine- and amphetamine-regulated transcript peptide and parvalbumin immunoreactivity in the hippocampus proper of the chinchilla

• Published in: Polish journal of veterinary sciences Vol. 23; no. 1; pp. 89 - 96
• Main Authors: Szalak, R, Matysek, M, Kukula-Koch, W, Rycerz, K, Zacharko-Siembida, A, Arciszewski, M B
• Format: Journal Article
• Language: English
• Published: Germany 01-03-2020
• Subjects: Animals; Chinchilla - metabolism; Hippocampus - physiology; Male; Nerve Tissue Proteins - metabolism; Parvalbumins - metabolism; Protein Transport - physiology; Tissue Distribution; chinchilla; dentate gyrus; memory; hippocampus; Ca2; PV; CART
• ISSN: 1505-1773; 2300-2557
• DOI: 10.24425/pjvs.2020.132752

This study set out to investigate, for the first time, the distribution and colocalization pattern of cocaine-and amphetamine-regulated transcript (CART) and one of the calcium binding- -proteins: parvalbumin (PV) in the chinchilla's hippocampus proper (HP). HP, consisting of Ammon's horn (CA) and the dentate gyrus (DG), is an important component of the limbic system, involved in learning and memory processes. CA showed a higher immunoreactivity of CART (-IR) compared to DG. CART-IR neurons were mainly observed in the molecular layer of DG and in the pyramidal layer of CA. CART-IR fibers were present in the granular layer; in the hilus numerous mossy fibers were detected, while in the molecular layer CART-IR fibers were not found. In all CA fields (CA1-CA3), CART-IR fibers were only present in the lacuno- sum-molecular layer. Immunofluorescence with double- labeling showed that only CART-IR cells stained positive for PV, whereas in CART-IR fibers there was no PV-positive reaction. Our research supplements missing knowledge about the distribution and colocalization pattern of CART with PV in the chinchilla's hippocampus, and also provides a better understanding of the similarities and differences among individuals of the same species and also with other mammals.