Development of DNA macroarrays for genome scanning of Ureaplasma parvum strains

Development of DNA macroarrays for genome scanning of Ureaplasma parvum strains

DNA macroarrays were developed on the basis of the known Ureaplasma parvum genome, which enabled rapid acquisition of the information on the changes in the microbial genome. For amplification of the PCR gene copies, 613 pairs of oligonucleotide primers were developed. Optimal conditions were determi...

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Bibliographic Details
Published in:Microbiology (New York) Vol. 78; no. 6; pp. 717 - 722
Main Authors: Klyubin, A. V, Selezneva, O. V, Chelysheva, V, Momynaliev, K. M
Format: Journal Article
Language:English
Published: Dordrecht Dordrecht : SP MAIK Nauka/Interperiodica 01-12-2009
SP MAIK Nauka/Interperiodica
Subjects:
Biomedical and Life Sciences
Experimental Articles
Life Sciences
Medical Microbiology
Microbiology
Ureaplasma parvum
hybridization
genome
DNA macroarrays
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Summary:DNA macroarrays were developed on the basis of the known Ureaplasma parvum genome, which enabled rapid acquisition of the information on the changes in the microbial genome. For amplification of the PCR gene copies, 613 pairs of oligonucleotide primers were developed. Optimal conditions were determined for immobilization of the PCR products on a Nylon membrane and for hybridization with U. parvum chromosomal DNA. The DNA macroarrays were used to compare the nucleotide sequences of the genomes of laboratory strains of U. parvum and U. urealyticum.
Bibliography:http://dx.doi.org/10.1134/S0026261709060083
ObjectType-Article-2
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0026-2617
1608-3237
DOI:10.1134/S0026261709060083