Protocorm development of Epidendrum fulgens (Orchidaceae) in response to different saline formulations and culture conditions

• Published in: Acta scientiarum. Biological sciences Vol. 36; no. 3; pp. 287 - 292
• Main Authors: Voges, Joana Gerent, Benevenuto, Rafael Fonseca, Fritsche, Yohan, Guerra, Miguel Pedro
• Format: Journal Article
• Language: English
• Published: Universidade Estadual de Maringá 20-08-2014
• Subjects: asymbiotic techique; culture medium; germination; seeds
• ISSN: 1679-9283; 1807-863X
• DOI: 10.4025/actascibiolsci.v36i3.21079

The asymbiotic technique of orchid seeds germination is an important method of mass production of seedlings. Studies on the best culture conditions for each species are important to obtain seedlings in less time and at lower costs. Current analysis evaluates different consistencies of culture medium, saline formulations and culture conditions on the germination rate and further development of protocorms of Epidendrum fulgens. After 45 days in culture the protocorms were classified into three categories of development. The liquid saline formulation of Murashige and Skoog (1962) (MS) provided the highest germination rate (83.5%), and the Knudson formulation (1946) the lowest (10.9%). The different consistencies or conditions or culture conditions did not affect the germination rate percentage, except the Knudson medium, which resulted in the highest rate in response to the gelled consistency. Protocorms cultured in liquid MS medium with or without agitation showed the fastest development.