Copper tolerance in Silene cucubalus: Subcellular distribution of copper and its effects on chloroplasts and plastocyanin synthesis

• Published in: Planta Vol. 167; no. 1; pp. 30 - 36
• Main Authors: Lolkema, P.C., Vooijs, R.
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01-01-1986; Springer
• Subjects: Animal and plant ecology; Animal, plant and microbial ecology; Autoecology; Biological and medical sciences; Cell walls; Chemical suspensions; Chlorophylls; Chloroplasts; Copper; Fundamental and applied biological sciences. Psychology; Leaves; Nutrient solutions; Plant roots; Plants; Plants and fungi; Population growth; Plastocyanin; Dicotyledones; Angiospermae; Tolerance; Spermatophyta; Biosynthesis; Silene; Copper; Caryophyllaceae; Heavy metal
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00446365

The uptake, translocation and subcellular distribution of copper as well as its effect on chloroplasts and plastocyanin synthesis were studied in a copper-sensitive and a copper-tolerant population of Silene cucubalus (L.) Wib. As a function of time, the copper concentration in roots of tolerant plants increased more slowly than that in roots of sensitive ones. Translocation to the shoot occurred more rapidly in tolerant plants than in sensitive ones. Although it was accumulated in leaf cells, copper was not accumulated in the chloroplasts of either sensitive or tolerant plants. Chlorophyll content was not affected by copper in tolerant plants, whereas sensitive plants became chlorotic. Plastocyanin synthesis was not enhanced as a result of high copper concentrations and no difference in plastocyanin content between tolerant and sensitive plants was detected. Measurements of copper in purified cell walls revealed that storage of the metal in cell-wall material does not play an important role in tolerance mechanism. Uptake characteristics, distribution and cytoplasmic detoxification of copper are discussed.