Rapid identification of biosurfactant-producing bacterial strains using a cell surface hydrophobicity technique

• Published in: Biotechnology techniques Vol. 11; no. 9; pp. 671 - 674
• Main Authors: PRUTHI, V, CAMEOTRA, S. S
• Format: Journal Article
• Language: English
• Published: London Chapman and Hall 01-09-1997
• Subjects: Biological and medical sciences; Biotechnology; Fundamental and applied biological sciences. Psychology; Methods. Procedures. Technologies; Others; Various methods and equipments; Screening; Production; Bacteria; Hydrophobicity; Technique; Surfactant; Cell surface
• ISSN: 0951-208X; 1573-6784
• DOI: 10.1023/A:1018411427192

Rapid identification of biosurfactant-producing bacterial strains was achieved by assaying cell surface hydrophobicity which had a direct correlation with biosurfactant production by Serratia marcescens, Pseudomonas aeruginosa, Bacillus pumilus, B. laterosporus, Acinetobacter calcoaceticus, Escherichia coli and Staphylococcus aureus. These properties namely, Hydrophobic Interaction Chromatography, Salt Aggregation Test, Bacterial Adherence To Hydrocarbon and adhesion to polystyrene by Replica Plate test, provide a simple means for identifying bacteria associated with the production of biosurfactants.