Multiplication of lavender (L. angustifolia) and lavandin (Lavandula x intermedia) in explant culture

• Published in: Agriculture Vol. 69; no. 1; pp. 1 - 12
• Main Authors: Gubišová, Marcela, Čičová, Iveta
• Format: Journal Article
• Language: English
• Published: Bratislava Sciendo 01-04-2023; De Gruyter Poland
• Subjects: Acclimatization; Agriculture; ex vitro acclimatization; Flowers & plants; Genotypes; Growth regulators; in vitro propagation; Lavandula angustifolia; Lavandula intermedia; lavandula x intermedia; Media; Nutrients; Oils & fats; Optimization; Petroleum production; Plantlets; Plants (botany); propagation; seedling production; Seeds; Segments; Shoots; Survivability
• ISSN: 1338-4376; 0551-3677; 1338-4376
• DOI: 10.2478/agri-2023-0001

Lavender plants are preferably propagated vegetatively. The aim of the work was to propose the composition of nutrient media for
multiplication of plants of
(var. Krajová and Beta) and
(var. Budrovka and Grosso). Explant cultures were initiated from apical or nodal segments. Apical segments were more suitable and regenerated the highest number of shoots in MS medium supplemented with 1 mg/L BAP with or without 0.1 mg/L IAA in all used varieties. Nodal segments are suitable for var. Budrovka, where the number of shoots was not statistically different between the apical or nodal segments and among the media tested. Shoot multiplication of
varieties was the most effective in the MS media with 0.5 or 1 mg/L BAP. For variety Grosso, the optimal medium was MS with 1 mg/L BAP, while var. Budrovka regenerated a similar number of shoots in all media supplemented with 0.5 or 1 mg/L BAP with or without 0.1 mg/L IAA. All genotypes rooted with the highest frequency on growth regulator-free medium with a half dose of MS salts. After transplanting into the soil and
acclimatization, plantlets survivability after 10 weeks was 78.9 – 87.7%, except for var. Beta with a lower survivability of 50%. The proposed procedures enable a rapid plant multiplication of varieties Budrovka, Grosso and Krajová. With the Beta variety, it is advisable to continue working on the optimization of nutrient media to verify whether the procedures can be further optimized.