Morphophysiology and cryopreservation of seeds of Dendrobium nobile Lindl. (Orchidaceae) at different stages of development

• Published in: Acta physiologiae plantarum Vol. 44; no. 3
• Main Authors: Kolomeitseva, G. L., Nikishina, T. V., Babosha, A. V., Ryabchenko, A. S., Vysotskaya, O. N.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-03-2022; Springer Nature B.V
• Subjects: Agriculture; Air flow; Biomedical and Life Sciences; Cryogenic storage; Cryopreservation; Dendrobium nobile; Developmental stages; Drying; Embryos; Freezing; Germination; Life Sciences; Liquid nitrogen; Plant Anatomy/Development; Plant Biochemistry; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Pollination; Rhizoids; Seeds; Short Communication; Staining; Storage; Cryogenic storage; Orchids; Vital dyes; Embryogenesis; Immature seeds; In vitro
• ISSN: 0137-5881; 1861-1664
• DOI: 10.1007/s11738-022-03372-z

Key message
The immature seeds of
Dendrobium nobile
acquire resistance to freezing and long-term storage in a cryobank at the age of 6 months after pollination.
Seed maturation of
Dendrobium nobile
Lindl to establish the optimal stage for long-term storage of immature seeds in liquid nitrogen was studied. Immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids. The maximum staining of embryos with vital dyes FDA and TTC occurs at 4 and 5 MAP that corresponds to the release of suspensor beyond the embryo sac. Embryo staining does not correlate with germination capacity and seed viability in cryogenic storage. The immature seeds acquire resistance to drying in airflow and cryostorage at the age of 6 MAP. 11% of seeds germinated after cryogenic storage at 6 MAP, and 81–94% at 7–10 MAP.