Tuning Zn(Ⅱ) selectivity by conjugating vitamin B6 cofactors over bovine serum albumin stabilized red-emitting silver nanoclusters

• Published in: Analytica chimica acta Vol. 1235; p. 340538
• Main Authors: Nakum, Rajanee, Upadhyay, Yachana, Sahoo, Suban K.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01-12-2022
• Subjects: Fluorescent silver nanoclusters; Fluorescent turn-on sensor; Vitamin B6 cofactors; Zn2; Fluorescent turn-on sensor; Fluorescent silver nanoclusters; Vitamin B6 cofactors; Zn2
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2022.340538

The red-emitting bovine serum albumin stabilized silver nanoclusters (BSA-AgNCs) were conjugated with the vitamin B6 cofactors pyridoxal 5′-phosphate (PLP) and pyridoxal (PL). The deconvoluted bands in XPS revealed that the conjugation occurred due to the formation of imine-linkage between the vitamin B6 cofactors and the BSA functionalized over BSA-AgNCs. The TEM images supported the formation of pseudo-spherical shaped nano-aggregates upon conjugating vitamin B6 cofactor PLP over BSA-AgNCs. An emission band appeared at 532 nm after the conjugation of PLP or PL over the surface of BSA-AgNCs. The in situ formed nano-assembly PLP_BSA-AgNCs and PL_BSA-AgNCs showed a significant fluorescent enhancement, respectively, at 490 nm and 481 nm in the presence of Zn2+. The complexation-induced fluorescence enhancement from PLP_BSA-AgNCs and PL_BSA-AgNCs was reversed by adding a strong chelating agent EDTA. In addition, the fluorescence of PL_BSA-AgNCs was irreversibly quenched with the appearance of an emission band at 423 nm in the presence of Hg2+. The analytical performance and sensing mechanism of the developed nanoprobes PLP_BSA-AgNCs and PL_BSA-AgNCs were discussed, and the highly selective and sensitive nanoprobe PLP_BSA-AgNCs was applied for the quantification of Zn2+ in real samples.
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•Red-emitting BSA-AgNCs was conjugated with the vitamin B6 cofactors PLP and PL.•TEM images supported the formation of flower-shaped nanoaggregates upon conjugation of vitamin B6 cofactors over BSA-AgNCs.•The in situ formed PLP_BSA-AgNCs and PL_BSA-AgNCs showed a significant fluorescent enhancement in the presence of Zn2+.•Highly sensitive nanoprobe PLP_BSA-AgNCs was applied for the quantification of Zn2+ in real samples.