Graphene Nanoplatelets-Based Aptamer Biochip for the Detection of Lipocalin-2

• Published in: IEEE sensors journal Vol. 19; no. 21; pp. 9592 - 9599
• Main Authors: Matassan, Nur Diyanah, Rizwan, Mohammad, Mohd-Naim, Noor Faizah, Tlili, Chaker, Ahmed, Minhaz Uddin
• Format: Journal Article
• Language: English
• Published: New York IEEE 01-11-2019; The Institute of Electrical and Electronics Engineers, Inc. (IEEE)
• Subjects: Biochips; Biosensors; Electrochemical aptasensor; Electrodes; Graphene; graphene nanoplatelets; Immune system; lipocalin; Organic chemistry; Proteins; screen-printed carbon electrode; Selectivity; Square waves; Target recognition; Voltammetry
• ISSN: 1530-437X; 1558-1748
• DOI: 10.1109/JSEN.2019.2927139

An electrochemical label-free aptasensor platform was developed by using amine-functionalized aptamer that was incorporated onto screen-printed carbon electrode (SPCE) modified with graphene nanoplatelets (GNPs) for the detection of lipocalin-2 (LCN-2) protein. The aptamers were covalently bound to the GNPs surface via 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) and N-hydroxysuccinimide (NHS) chemistry and were used as the molecular recognition element for the target protein. Successful interaction between aptamer and LCN-2 protein was ascertained via electrochemical probe, ferro-, and ferri-cyanide {Fe(CN) 6 3-/4- } and monitored by means of square wave voltammetry (SWV) whilst layer-by-layer fabrication study was done using cyclic voltammetry (CV). The developed LCN-2 aptasensor achieved sub-picomolar detection of the protein, with the detection limit determined as 0.07 pg ml -1 , and a dynamic linear range of 0.1 to 10 pg ml -1 . Fabricated aptasensor successfully demonstrated high selectivity, stability, and reproducibility as well as displaying potential for the in vitro detection of LCN-2 in real serum samples.