Intraindividual Temporal miRNA Variability in Serum, Plasma, and White Blood Cell Subpopulations

Intraindividual Temporal miRNA Variability in Serum, Plasma, and White Blood Cell Subpopulations

Blood microRNAs (miRNAs) are ideal biomarkers, and blood derivatives are often collected in the scope of miRNA research projects. However, knowledge of temporal variations of miRNAs in healthy individuals is lacking. In this study, miRNA variability was measured over a 1-year period in different blo...

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Bibliographic Details
Published in:Biopreservation and biobanking Vol. 14; no. 5; p. 390
Main Authors: Ammerlaan, Wim, Betsou, Fay
Format: Journal Article
Language:English
Published: United States 01-10-2016
Subjects:
Biomarkers - blood
Female
Gene Expression Profiling
Genetic Variation
Humans
Leukocytes - chemistry
Male
MicroRNAs - analysis
MicroRNAs - blood
Middle Aged
Plasma - chemistry
Serum - chemistry
Specimen Handling - standards
blood cells
miRNA
plasma
serum
stability
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Summary:Blood microRNAs (miRNAs) are ideal biomarkers, and blood derivatives are often collected in the scope of miRNA research projects. However, knowledge of temporal variations of miRNAs in healthy individuals is lacking. In this study, miRNA variability was measured over a 1-year period in different blood derivatives, collected every 2-3 months from two healthy donors. There is a continuum of intraindividual temporal variability, with particularly stable (coefficient of variation [CV] <20%-30%) and particularly unstable (CV >100%-130%) miRNAs in serum, plasma, and specific white blood cell subpopulations. The temporal intraindividual variability of miRNAs should be taken into consideration in experimental design of biospecimen collections and validation of diagnostic biomarkers.
ISSN:1947-5543
DOI:10.1089/bio.2015.0125