Determination of plasma cytidine deaminase activity by HPLC

• Published in: Biomedical chromatography Vol. 2; no. 4; p. 148
• Main Authors: Richards, D A, Sherwood, R A, Ndebele, D, Rocks, B F
• Format: Journal Article
• Language: English
• Published: England 1987
• Subjects: Chromatography, High Pressure Liquid; Cytidine Deaminase - blood; Humans; Nucleoside Deaminases - blood; Uridine - blood
• ISSN: 0269-3879
• DOI: 10.1002/bmc.1130020404

Cytidine deaminase is an enzyme of nucleic acid metabolism, the measurement of which has been proposed as a useful test for the early detection of pre-eclamptic toxaemia in pregnancy. The enzyme converts the nucleoside cytidine to uridine, with the release of ammonia, and it is the measurement of this latter compound that forms the basis of the conventional methods for the assay of cytidine deaminase. The low activity of the enzyme requires long incubation times, which in turn increase the possibility of contamination by exogenous ammonia. We have developed a new method for determining cytidine deaminase activity, utilising high performance liquid chromatography to measure the production of uridine. This method uses much shorter incubation times and is unaffected by ammonia contamination. This paper describes the development of the method and its comparison with the established assay. The relative merits of each are discussed. Finally, the adaptation of incubation and chromatographic conditions, in order to measure other enzymes of nucleic acid metabolism which are of clinical interest, is briefly mentioned.