Decolorization of safranin using Fissidens species and its ecotoxicological assessments: An in vitro and in silico approach

• Published in: Environmental research Vol. 211; p. 113108
• Main Authors: Kiruthika, T., Poonkothai, M., Kalaiarasi, K., Ajarem, Jamaan S., Allam, Ahmed A., Khim, Jong Seong, Sudhakar, C., Selvankumar, T., Alaguprathana, M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01-08-2022
• Subjects: Adsorption; Characterization; Fissidens species; In silico study; Optimization; Toxicological assessments; Characterization; Toxicological assessments; Fissidens species; Adsorption; Optimization; In silico study
• ISSN: 0013-9351; 1096-0953
• DOI: 10.1016/j.envres.2022.113108

Decolorization of safranin was investigated using Fissidens species in a batch system under optimized conditions. The decolorization efficiency was improved by optimizing the conditions such as initial pH (3–9), temperature (25–45 °C), initial dye concentration (10–50 mg/L), biosorbent dosage (100–500 mg/L) and contact time (1–6 days). Maximum decolorization (95%) was recorded at initial pH of 6 with dye concentration of 20 mg/L, biosorbent dosage of 200 mg/L at 30 °C and contact time of 2 days. Desorption studies revealed 0.1 N NaOH as the best desorbing agent with 92% recovery on third day. Experimental data well fitted to Langmuir isotherm and Pseudo-second order kinetic model. The negative values of ΔGo and positive value of ΔSo and ΔHo indicates that the reaction is spontaneous, favorable and endothermic. The biosorbent - dye interactions were confirmed using UV–Vis, FT-IR, XRD and FE-SEM with EDX studies. The detoxified nature of the dye degraded metabolites was confirmed by the significant growth of green gram. The color fastness and color strength of the fabrics dyed using Fissidens species treated dye solution were compared with the tap water dyed fabrics which indicated the reuse potential of treated water in textile sector. The decolorization efficiency was further confirmed through in silico approach, where safranin well docked with the active sites of Photosystem II protein D1 of the Fissidens species. Thus, the present study proves that Fissidens species is a promising biosorbent for safranin decolorization and will lay a platform for the control and management of environmental pollution.
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