Soluble polypeptides from meristematic and mature cells of Allium cepa L. roots

• Published in: Planta Vol. 142; no. 2; pp. 147 - 151
• Main Authors: Navarrete, M.H, Bernavbeu, C
• Format: Journal Article
• Language: English
• Published: Germany Springer-Verlag 1978
• Subjects: Cell growth; Cell lines; Cellular differentiation; Developmental biology; Electrophoresis; Gels; Meristems; Molecular weight; onions; Plant roots; Radioactive decay
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/BF00388205

By using electrophoresis on SDS polyacrylamide gels we have studied the soluble protein fraction from a section of the meristem, where most cells are in the division last cycle, and from the mature region of Allium cepa L. roots. In order to estimate the apparent rate of synthesis of these polypeptides we labeled a series of roots with [14C]leucine and another with [3H]leucine. Coelectrophoresis was carried out by using polypeptides from both regions, their mol.wt. being between 20,000 and 100,000 daltons. The results show that most of the polypeptides in the soluble fraction are constantly present in a cell throughout its development. These constant polypeptides are synthesized at a high rate in the meristematic region. In the mature cells these polypeptides show only a low labeling rate, while a small number of specific polypeptides appear to have a very high rate of metabolism.