Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing

• Published in: Conservation genetics resources Vol. 6; no. 1; pp. 185 - 187
• Main Authors: Jackson, Alexis M, Munguía-Vega, Adrian, Lain, Avian S, Stokes, Simon A, Williams, Aneese J, Bernardi, Giacomo
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01-03-2014; Springer Netherlands; Springer Nature B.V
• Subjects: alleles; Animal Genetics and Genomics; Biodiversity; Biomedical and Life Sciences; chemistry; Conservation Biology/Ecology; Ecology; Evolutionary Biology; genomic libraries; Genotyping; Heterozygosity; Life Sciences; loci; Microsatellite Letters; microsatellite repeats; Mycteroperca rosacea; Plant Genetics and Genomics; sequence analysis; Threatened species; titanium; Gulf of California; Mexico; Leopard grouper; Microsatellites; Fisheries management; Conservation
• ISSN: 1877-7252; 1877-7260
• DOI: 10.1007/s12686-013-0044-0

Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975.