Plantago australis Hydroethanolic Extract-Loaded Formulations: Promising Dressings for Wound Healing

• Published in: Revista brasileira de farmacognosia Vol. 31; no. 1; pp. 91 - 101
• Main Authors: Reinhardt, Luiza Steffens, Henn, Jeferson Gustavo, Morás, Ana Moira, de Moura Sperotto, Nathalia Denise, Ferro, Matheus Bernardes, Cao, Zhi, Roehe, Adriana Vial, Petry, Adriana Ubirajara Silva, Nugent, Michael, Moura, Dinara Jaqueline
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01-02-2021
• Subjects: Medicine; Original Article; Pharmacy; Electrospinning; Nanofiber; Polyvinyl alcohol; Wound dressing; Ointment
• ISSN: 1981-528X; 1981-528X
• DOI: 10.1007/s43450-021-00126-9

The productions of wound dressings involve three key goals: wound protection, suitable environment, and acceleration of the healing process. This study was based on our previous findings of a
Plantago australis
Lam., Plantaginaceae, hydroethanolic extract and its pronounced wound healing activity, leading to the development of wound dressings containing the extract. The hydroethanolic extract was produced by an ultrasound method, using leaves followed by HPLC characterization. A nanofiber containing the extract was produced by using polyvinyl alcohol and electrospinning and had its mechanical and physical properties characterized. The hydroethanolic extract was found stable after electrospun and the nanofiber provided a continuous drug release. An ointment containing the extract was produced in lanolin:vaseline base by spatulation.
In vitro
and
in vivo
wound healing activities were assessed by 3D cell culture and by inflicting wounds in Wistar rats and placing the dressings up to 14 days, respectively.
In vitro
proliferation assay showed that exposure to hydroethanolic extract nanofiber increases the size of the spheroid (sum of the spheroid area and cell proliferation area) compared to the extract and blank nanofiber.
In vivo
testing indicated enhanced wound healing efficacy of animals treated with the hydroethanolic extract nanofiber and ointment when compared to the hydrogel containing the extract and blank formulations. Thus, these dressings could be used to maximize the healing process, improving the treatment outcome.