Reciprocal interaction between melatonin receptors (Mel1a, Mel1b, and Mel1c) and androgen receptor (AR) expression in immunoregulation of a seasonally breeding bird, Perdicula asiatica: Role of photoperiod

• Published in: Journal of photochemistry and photobiology. B, Biology Vol. 122; pp. 52 - 60
• Main Authors: Yadav, S.K., Haldar, C.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05-05-2013
• Subjects: Androgen receptor; androgen receptors; Aves; breeding; Immunity; immunomodulation; interleukin-2; leukocyte count; melatonin; Melatonin receptor; messenger RNA; Perdicula asiatica; photobiology; photochemistry; Photoperiod; Quail; reverse transcriptase polymerase chain reaction; spleen; testes; testosterone; tumor necrosis factor-alpha; wild birds; Photoperiod; Quail; Immunity; Melatonin receptor; Androgen receptor
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2013.03.005

•We are reporting the photoperiodic regulation of Mel1a Mel1b, Mel1c and AR.•Expression of Mel1a and Mel1b in spleen was high in SD bird as compared to LD bird.•Our RT-PCR results supports differentially localised mRNA Mel1b and Mel1c.•We found compensatory mechanism between reproduction and immunity in this bird.
Light is the major environmental stimulus affecting behaviour and physiology of avian species. Our study elaborates the photoperiodic regulation of melatonin (Mel1a, Mel1b, and Mel1c) and androgen receptor (AR) to elucidate its reciprocal interaction in regulation of general immunity in tropical wild bird, Perdicula asiatica. Effect of different photoperiodic exposures such as continuous light (LL), continuous dark (DD), long days (LD; 16h light/day), short days (SD; 10h light/day) and normal day length (NDL) was accessed both on cellular and humoral immune parameters like per cent stimulation ratio (%SR), total leukocyte count (TLC), leukocyte count (LC), plasma interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), spleen and gonad weight, plasma melatonin, and testosterone level as well as their receptor expression on spleen and testis. Expression of melatonin receptor, Mel1a and Mel1b in spleen was high in SD experiencing bird as compared to LD birds. In all photoperiodic groups, AR expression was upregulated in spleen. In addition, our reverse transcription polymerase chain reaction (RT-PCR) results support differentially localized mRNA Mel1b and Mel1c expression in spleen and testis. In sum, photoperiodically modulated level of melatonin via reciprocal regulation of Mel1a, Mel1b, and Mel1c, and AR in spleen as well as in testis modulates immunity, suggesting a compensatory mechanism between reproduction and immunity in a seasonally breeding bird, P. asiatica.