Serinibacter arcticus sp. nov., isolated from a thawing ancient ice wedge

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 2; pp. 929 - 934
• Main Authors: Filippova, Svetlana N, Surgucheva, Natalya A, Detkova, Ekaterina N, Rakitin, Andrey L, Beletsky, Aleksey V, Grouzdev, Denis S, Kolganova, Tatyana V, Mulyukin, Andrey L
• Format: Journal Article
• Language: English
• Published: England 01-02-2020
• Subjects: Actinobacteria - classification; Actinobacteria - isolation & purification; Bacterial Typing Techniques; Base Composition; Cell Wall - chemistry; DNA, Bacterial - genetics; Fatty Acids - chemistry; Glycolipids - chemistry; Ice; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phospholipids - chemistry; Phylogeny; Pigmentation; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Siberia; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; Siberia; actinobacteria; Serinibacter arcticus sp. nov; ice wedge; Mammoth Mountain
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003848

A novel actinobacterium, strain K3-2
, was isolated in pure culture from a thawing ancient ice wedge at Mammoth Mountain (Eastern Siberia, Russia). Colonies of strain K3-2
were yellowish orange; cells had the fine structure typical of Gram-positive bacteria, were non-motile short rods and were non-spore-forming. Strain K3-2
was mesophilic (optimum growth at 28 °С), but capable of growing at 4 °С. The cell-wall peptidoglycan of strain K3-2
contained lysine (the diagnostic diamino acid), glutamic acid, alanine, ornithine, glycine and serine. The polar lipids were phosphatidylglycerol, lysophosphatidylserine, three unidentified phospholipids and glycolipids. The major fatty acids were anteiso-C
and C
. The only menaquinone detected was MK-8(H
). 16S rRNA gene analysis indicated that strain K3-2
belongs to the genus
. The closest taxonomically described relatives were
Kis4-28
and
PS-14-7
, with 97.20 and 97.20 % 16 s rRNA gene sequence similarity, respectively. The average nucleotide identity value of the whole genome sequence between strain K3-2
and
Kis4-28
was 78.9 %. DNA-DNA relatedness values between strain K3-2
and
DSM 21801
(=Kis4-28
) and
VKPM Ac 2044
(=PS-14-7
) were 41 and 47 %. Thus, strain K3-2
represents a novel species of the genus
for which the name
sp. nov. is proposed. The type strain is K3-2
(DSM 103859
=VKM Ас-2719
).