Simple repetitive sequences are associated with differentiation of the sex chromosomes in the guppy fish

Hybridization of restriction enzyme-digested genomic guppy Poecilia reticulata , Poeciliidae) DNA with the oligonucleotide probe (GACA) sub(4) revealed a male-specific simple tandem repeat locus, which defines the Y chromosome in outbred populations. The related (GATA) sub(4) probe identified certai...

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Bibliographic Details
Published in:	Journal of molecular evolution Vol. 30; no. 5; pp. 456 - 462
Main Authors:	NANDA, I, FEICHTINGER, W, SCHMID, M, SCHRÖDER, J. H, ZISCHLER, H, EPPLEN, J. T
Format:	Journal Article
Language:	English
Published:	New York, NY Springer 01-01-1990
Subjects:	Biological and medical sciences Biological evolution Freshwater Fundamental and applied biological sciences. Psychology Genetics of eukaryotes. Biological and molecular evolution Poecilia reticulata Freshwater environment Vertebrata Heterochromatin Repeated sequence Pisces DNA Fingerprint method Sex chromosome Cytogenetics Poecilia reticulata Y-Chromosome Karyotype
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Summary:	Hybridization of restriction enzyme-digested genomic guppy Poecilia reticulata , Poeciliidae) DNA with the oligonucleotide probe (GACA) sub(4) revealed a male-specific simple tandem repeat locus, which defines the Y chromosome in outbred populations. The related (GATA) sub(4) probe identified certain males with the red color phenotype. In contrast only in two out of eight laboratory guppy strains was the typical (GACA) sub(4) band observed. By specific staining of the constitutive heterochromatin one pair of chromosomes could also be identified as the sex chromosomes, confirming the XX/XY mechanism of sex determination.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0022-2844 1432-1432
DOI:	10.1007/BF02101117