A Reactor-Type Biosensor Based on Rhodococcus erythropolis HL PM-1 Cells for Detecting 2,4-Dinitrophenol

• Published in: Applied biochemistry and microbiology Vol. 38; no. 5; pp. 500 - 505
• Main Authors: Kitova, A E, Kuvichkina, T N, Il'yasov, P V, Arinbasarova, A Yu, Reshetilov, A N
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Nature B.V 01-09-2002
• Subjects: 2,4-Dinitrophenol; Agar; Bacteria; Biosensors; calcium alginate; Cellulose; Ceramics; Gels; Inactivation; Powder; Reactors; Rhodococcus erythropolis
• ISSN: 0003-6838; 1608-3024
• DOI: 10.1023/A:1019989023130

A model of a reactor-type biosensor based on the Rhodococcus erythropolis HL PM-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-DNP). The effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. In the case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-DNP detection was 20 μM and the time of single analysis, the biosensor recovery included, was 30-50 min. In the continuous detection mode, the biosensor response was maintained at a stable level without biosensor inactivation for ten days. The biosensor can be used as an element of a complex analytical system for detecting nitroaromatic compounds in samples.[PUBLICATION ABSTRACT]