The role of remodeling complexes CHD1 and ISWI in spontaneous and UV-induced mutagenesis control in yeast Saccharomyces cerevisiae

• Published in: Russian journal of genetics Vol. 53; no. 2; pp. 195 - 201
• Main Authors: Evstiukhina, T. A., Alekseeva, E. A., Fedorov, D. V., Peshekhonov, V. T., Korolev, V. G.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-02-2017
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Biomedicine; Genetics of Microorganisms; Human Genetics; Microbial Genetics and Genomics; repair; transcription; chromatin; genomic stability; gene expression
• ISSN: 1022-7954; 1608-3369
• DOI: 10.1134/S1022795417010057

Chromatin remodulators are special multiprotein machines capable of transforming the structure, constitution, and positioning of nucleosomes on DNA. Biochemical activities of remodeling complexes CHD1 and ISWI from the SWI2/SNF2 family are well established. They ensure correct positioning of nucleosomes along the genome, which is probably critical for genome stability, in particular, after action of polymerases, repair enzymes, and transcription. In this paper, we show that single mutations in genes
ISW1
,
ISW2
, and
CHD1
weakly affect repair and mutagenic processes in yeast cells. At the same time, there are differences in the effect of these mutations on spontaneous mutation levels, which indicates certain specificity of action of protein complexes ISW1, ISW2, and CHD1 on expression of different genes that control repair and mutation processes in yeast.