Determination of the activity of bacteriolytic enzymes and measurement of their sorption in the system of living cells of Lactobacillus plantarum

• Published in: Moscow University chemistry bulletin Vol. 70; no. 6; pp. 292 - 297
• Main Authors: Matolygina, D. A., Osipova, H. E., Smirnov, S. A., Belogurova, N. G., Eremeev, N. L., Tishkov, V. I., Levashov, A. V., Levashov, P. A.
• Format: Journal Article
• Language: English
• Published: New York Allerton Press 01-11-2015
• Subjects: Chemistry; Chemistry and Materials Science; Chemistry/Food Science; chicken egg lysozyme; bacteriolytic activity
• ISSN: 0027-1314; 1935-0260
• DOI: 10.3103/S002713141506005X

A turbidimetric method for determining the activity of bacteriolytic enzymes against living
Lactobacillus plantarum
cells as a substrate is developed. A technique for measuring the sorption of an enzyme on living cells in an experiment for activity determination is described, with chicken egg lysozyme being used as a standard model enzyme. The correctness of the calculations of the kinetic parameters is proved by counting the colony-forming units (CFUs). The physical and chemical parameters of the sorption of the enzyme on the cells are calculated and the correctness of the obtained equilibrium desorption constants is confirmed.