Evaluation of tropical seaweeds as feedstock for bioethanol production

• Published in: International journal of environmental science and technology (Tehran) Vol. 15; no. 5; pp. 977 - 992
• Main Authors: Hessami, M. J., Phang, S.-M., Salleh, A., Rabiei, R.
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01-05-2018
• Subjects: Aquatic Pollution; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Science and Engineering; Original Paper; Soil Science & Conservation; Waste Water Technology; Water Management; Water Pollution Control; Fermentation inhibitors; Bioethanol; Dilute acid hydrolysis; Tropical seaweeds
• ISSN: 1735-1472; 1735-2630
• DOI: 10.1007/s13762-017-1455-3

Limited resources of freshwater and decreasing fossil fuel resources are two main reasons to consider the ocean as a huge resource for producing food, feed, fertilizer and feedstock for fuel. In this study, twenty-nine tropical seaweeds (11 green, 10 red and 8 brown seaweeds) collected in Malaysia were assessed as potential feedstock for bioethanol production. Total carbohydrate content ranged from 12.16 to 71.22% dry weight (DW) with total reducing sugar content ranging from 5.17 to 34.12% DW. During hydrolysis using dilute sulphuric acid, the dominant fermentation inhibitors were 5-hydroxymethylfurfural and phenolic compounds. Overliming was found to reduce the content of fermentation inhibitors by up to 79%. The red seaweeds,
Kappaphycus alvarezii
(Doty) Doty ex P.C.Silva and
Gracilaria manilaensis
Yamamoto and Trono, were selected for optimization of saccharification and fermentation of the hydrolysate, because they had the highest carbohydrate contents and are commercially cultivated. The most suitable dilute acid conditions obtained in present study was sulphuric acid (2.5%, w v
−1
) treatment at 121 °C for 40 min that produced 0.29 and 0.34 g g
−1
DW reducing sugar for
K. alvarezii
and
G. manilaensis,
respectively. Fermentation of the hydrolysates with
Saccharomyces cerevisiae
produced bioethanol yields of 20.90 g L
−1
(71.0% of theoretical yield) for
K. alvarezii
and 18.16 g L
−1
(67.9% theoretical yield) for
G. manilaensis.