Microfluidic toner-based analytical devices: disposable, lightweight, and portable platforms for point-of-care diagnostics with colorimetric detection

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1256; p. 85
• Main Authors: Oliveira, Karoliny Almeida, de Souza, Fabrício Ribeiro, de Oliveira, Cristina Rodrigues, da Silveira, Lucimeire Antonelli, Coltro, Wendell Karlos Tomazelli
• Format: Journal Article
• Language: English
• Published: United States 2015
• Subjects: Antibodies, Viral - blood; Biological Assay; Blood Glucose - analysis; Cell Phone; Cholesterol - blood; Colorimetry - methods; Dengue - diagnosis; Dengue - virology; Dengue Virus - isolation & purification; Developing Countries; Disposable Equipment; Humans; Microfluidic Analytical Techniques - instrumentation; Microtechnology; Point-of-Care Systems; Polyesters - chemistry; Printing - methods; Proteins - analysis; Telemedicine - instrumentation; Telemedicine - methods; Triglycerides - blood
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-2172-0_6

This chapter describes the development of microfluidic toner-based analytical devices (μTADs) to perform clinical diagnostics using a scanner or cell-phone camera. μTADs have been produced in a platform composed of polyester and toner by the direct-printing technology (DPT) in a matter of minutes. This technology offers simplicity and versatility, and it does not require any sophisticated instrumentation. Toner-based devices integrate the current generation of disposable analytical devices along paper-based chips. The cost of one μTAD has been estimated to be lower than $0.10. In addition, these platforms are lightweight and portable thus enabling their use for point-of-care applications. In the last 5 years, great efforts have been dedicated to spread out the use of μTADs in bioassays. The current chapter reports the fabrication of printed microplates and integrated microfluidic toner-based devices for dengue diagnostics and rapid colorimetric assays with clinically relevant analytes including cholesterol, triglycerides, total proteins, and glucose. The use of μTADs associated with cell-phone camera may contribute to the health care, in special, to people housed in developing regions or with limited access to clinics and hospitals.