Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand

Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand

The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. ins...

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Published in:Medical mycology (Oxford) Vol. 57; no. 4; pp. 519 - 522
Main Authors: Worasilchai, N, Leelahavanichkul, A, Permpalung, N, Kuityo, C, Phaisanchatchawan, T, Palaga, T, Reantragoon, R, Chindamporn, A
Format: Journal Article
Language:English
Published: England 01-06-2019
Subjects:
Antibodies, Fungal - blood
Antigens, Fungal - immunology
Enzyme-Linked Immunosorbent Assay - methods
Humans
Immunoglobulin G - blood
Pythiosis - diagnosis
Pythium - immunology
Sensitivity and Specificity
Serologic Tests - methods
Thailand
Thailand
Pythium insidiosum
Serological diagnosis
Antigenic differences
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Abstract The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P < .0001). ELISA with antigen from human source might be more proper diagnosis test.
AbstractList The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P < .0001). ELISA with antigen from human source might be more proper diagnosis test.
The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P &lt; .0001). ELISA with antigen from human source might be more proper diagnosis test.
Author Phaisanchatchawan, T
Leelahavanichkul, A
Palaga, T
Kuityo, C
Worasilchai, N
Reantragoon, R
Permpalung, N
Chindamporn, A
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  surname: Worasilchai
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  fullname: Leelahavanichkul, A
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  organization: Division of Infectious Diseases, Duke University Medical Center, Durham, North Carolina, USA
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  organization: Interdisciplinary Program, Medical Microbiology, Graduate school, Chulalongkorn University, Bangkok, Thailand
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  surname: Reantragoon
  fullname: Reantragoon, R
  organization: Center of Excellence in Immunology and Immune-mediated diseases, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
– sequence: 8
  givenname: A
  surname: Chindamporn
  fullname: Chindamporn, A
  organization: Division of Mycology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
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Keywords Pythium insidiosum
Serological diagnosis
Antigenic differences
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SubjectTerms Antibodies, Fungal - blood
Antigens, Fungal - immunology
Enzyme-Linked Immunosorbent Assay - methods
Humans
Immunoglobulin G - blood
Pythiosis - diagnosis
Pythium - immunology
Sensitivity and Specificity
Serologic Tests - methods
Thailand
Title Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand
URI https://www.ncbi.nlm.nih.gov/pubmed/30165659
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