Antigen host response differences between the animal-type strain and human-clinical Pythium insidiosum isolates used for serological diagnosis in Thailand

• Published in: Medical mycology (Oxford) Vol. 57; no. 4; pp. 519 - 522
• Main Authors: Worasilchai, N, Leelahavanichkul, A, Permpalung, N, Kuityo, C, Phaisanchatchawan, T, Palaga, T, Reantragoon, R, Chindamporn, A
• Format: Journal Article
• Language: English
• Published: England 01-06-2019
• Subjects: Antibodies, Fungal - blood; Antigens, Fungal - immunology; Enzyme-Linked Immunosorbent Assay - methods; Humans; Immunoglobulin G - blood; Pythiosis - diagnosis; Pythium - immunology; Sensitivity and Specificity; Serologic Tests - methods; Thailand; Thailand; Pythium insidiosum; Serological diagnosis; Antigenic differences
• ISSN: 1369-3786; 1460-2709
• DOI: 10.1093/mmy/myy072

The detection of Pythium insidiosum-specific-immunoglobulin-G antibody (Pi-Ab) with enzyme-linked immunosorbent assay (ELISA) test depends on the source of antigen. In this study, the Pi-Ab levels in 140 serum samples from patients with pythiosis were evaluated by ELISA using antigens from 10 P. insidiosum clinical isolates in comparison with antigen from the equine-standard-type strain. The ELISA values (EVs), calculated from antibody levels from serum of patients with pythiosis or other infections versus healthy controls, were significantly higher in the test with clinical-isolates antigen than the standard-equine-type strain (6.0 ± 2.6 vs 4.0 ± 1.7, respectively; P < .0001). ELISA with antigen from human source might be more proper diagnosis test.