Proteome- and metabolome-level changes during early stages of clubroot infection in Brassica napus canola

• Published in: Molecular omics Vol. 20; no. 4; p. 265
• Main Authors: Adhikary, Dinesh, Mehta, Devang, Kisiala, Anna, Basu, Urmila, Uhrig, R Glen, Emery, Rj Neil, Rahman, Habibur, Kav, Nat N V
• Format: Journal Article
• Language: English
• Published: England 07-05-2024
• Subjects: Brassica napus - genetics; Brassica napus - metabolism; Brassica napus - parasitology; Disease Resistance - genetics; Metabolome; Metabolomics - methods; Plant Diseases - genetics; Plant Diseases - parasitology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - metabolism; Plasmodiophorida; Proteome - metabolism; Proteomics - methods; Tandem Mass Spectrometry
• ISSN: 2515-4184
• DOI: 10.1039/d3mo00210a

Clubroot is a destructive root disease of canola (
L.) caused by
Woronin. Despite extensive research into the molecular responses of
to
, there is limited information on proteome- and metabolome-level changes in response to the pathogen, especially during the initial stages of infection. In this study, we have investigated the proteome- and metabolome- level changes in the roots of clubroot-resistant (CR) and -susceptible (CS) doubled-haploid (DH)
lines, in response to
pathotype 3H at 1-, 4-, and 7-days post-inoculation (DPI). Root proteomes were analyzed using nanoflow liquid chromatography coupled with tandem mass spectrometry (nano LC-MS/MS). Comparisons of pathogen-inoculated and uninoculated root proteomes revealed 2515 and 1556 differentially abundant proteins at one or more time points (1-, 4-, and 7-DPI) in the CR and CS genotypes, respectively. Several proteins related to primary metabolites (
, amino acids, fatty acids, and lipids), secondary metabolites (
, glucosinolates), and cell wall reinforcement-related proteins [
, laccase, peroxidases, and plant invertase/pectin methylesterase inhibitors (PInv/PMEI)] were identified. Eleven nucleotides and nucleoside-related metabolites, and eight fatty acids and sphingolipid-related metabolites were identified in the metabolomics study. To our knowledge, this is the first report of root proteome-level changes and associated alterations in metabolites during the early stages of
infection in
.