S-metolachlor promotes oxidative stress in green microalga Parachlorella kessleri - A potential environmental and health risk for higher organisms

• Published in: The Science of the total environment Vol. 637-638; pp. 41 - 49
• Main Authors: Špoljarić Maronić, Dubravka, Štolfa Čamagajevac, Ivna, Horvatić, Janja, Žuna Pfeiffer, Tanja, Stević, Filip, Žarković, Neven, Waeg, Georg, Jaganjac, Morana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-10-2018
• Subjects: 4-Hydroxy-2-nonenal; Acetamides - toxicity; Aldehydes; Antioxidants; Chlorophyta - drug effects; Chlorophyta - physiology; Lipid Peroxidation; Microalgae - drug effects; Microalgae - physiology; Oxidative Stress; Parachlorella kessleri; Reactive oxygen species; S-metolachlor; Water Pollutants, Chemical - toxicity; NADPH; Reactive oxygen species; O2; Lipid peroxidation; APX; BBM; H2O2; PHE; Antioxidants; BSA; Parachlorella kessleri; HNE-His; OH; Chla; Chlb; GSH; DCFH; AA; PBS; 4-Hydroxy-2-nonenal; DNPH; OD750; PUFA; S-MET; DCF; GAE; GR; EDTA; DCFH-DA; ROO; 1O2; Car; S-metolachlor; CAT; ROS; HBSS; RO; TEM; HNE
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2018.04.433

The estimation of the toxic influences of herbicide products on non-target aquatic organisms is essential for evaluation of environmental contamination. We assessed the effects of the herbicide S-metolachlor (S-MET) on unicellular green microalga Parachlorella kessleri during 4–72 in vitro exposure to concentrations in the range 2–200μg/L. The results have shown that S-MET had a significant effect on algae, even in doses 10 and 20 times lower than the EC50 values obtained for P. kessleri (EC50-72h=1090μg/L). It generates reactive oxygen species in algae, decreases their growth and photosynthetic pigment concentration, changes their ultrastructure and alters the cellular antioxidant defence capacities. The levels of protein adducts with the reactive aldehyde 4-hydroxy-2-nonenal (HNE), the end-product of lipid peroxidation, were significantly elevated in S-MET treated cells revealing the insufficient effectiveness of P. kessleri antioxidant mechanisms and persistent lipid peroxidation. Since algae are fundamental aquatic food component, the damaged algal cells, still capable of dividing while having persistently increased content of HNE upon S-MET contamination could represent an important environmental toxic factor that might further affect higher organisms in the food chain.
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•Oxidative stress response was assessed in P. kessleri following S-MET exposure.•S-MET alters algal growth and antioxidant mechanisms inducing lipid peroxidation.•HNE-protein adducts accumulate in P. kessleri during longer S-MET treatment.•Algae with increased HNE content could represent environmental toxic factor.