Anti-Biofilm and Hemolytic Effects of Cymbopogon citratus (Dc) Stapf Essential Oil

• Published in: Pesquisa brasileira em odontopediatria e clínica integrada Vol. 19; no. 1; pp. 1 - 10
• Main Authors: Silva, Naiana Braga da, Rangel, Marianne de Lucena, Castro, Ricardo Dias de, Lima, Jefferson Muniz de, Castellano, Lúcio Roberto Cançado, Valença, Ana Maria Gondim, Cavalcanti, Alessandro Leite
• Format: Journal Article
• Language: English
• Published: Associação de Apoio à Pesquisa em Saúde Bucal 2019
• Subjects: DENTISTRY, ORAL SURGERY & MEDICINE; Biofilms; Drug Evaluation, Preclinical; Plants, Medicinal; Cymbopogon
• ISSN: 1519-0501; 1983-4632
• DOI: 10.4034/PBOCI.2019.191.103

Abstract Objective: To perform chemical analysis and to evaluate the anti-biofilm and hemolytic effect of the essential oil of Cymbopogon citratus. Material and Methods: Gaseous chromatography coupled to mass spectrometer was performed for chemical characterization of the essential oil. To verify the antimicrobial action, the Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and Minimum Fungicidal Concentration (MFC) were determined. From MIC, MBC and MFC data, concentrations were established to verify the anti-biofilm effect and for the hemolysis test on human erythrocytes. A multispecies biofilm was developed in vitro and mouthwash applications were simulated to determine the inhibition of biofilm formation or its removal. Results were analyzed through ANOVA statistical test, complemented by the Tukey test, considering a significance level of 5% Results: The major component of the essential oil is citral. MIC verified for Streptococcus mutans was 1mg / mL, while for Candida albicans, it was 125 μg/mL, presenting microbicidal effect for both microorganisms tested. The essential oil was able to inhibit biofilm formation (p<0.001), presenting non-toxic hemolysis percentage in concentration below 500 μg/mL Conclusion: The essential oil of Cymbopogon citratus is antimicrobial, antibiofilm and non-toxic to human erythrocytes, representing a natural product with potential for use in Dentistry.