Inhibition of Protein Synthesis by RA-VII

Inhibition of Protein Synthesis by RA-VII

The antitumor natural product RA-VII has been evaluated as an inhibitor of protein synthesis in vitro. Complete inhibition of protein synthesis in rabbit reticulocyte lysates is observed with 5 μM RA-VII. Mechanistic studies using purified elongation factors and ribosomes identify RA-VII as a peptid...

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Bibliographic Details
Published in:Bioorganic chemistry Vol. 23; no. 4; pp. 460 - 470
Main Authors: Sirdeshpande, B.V., Toogood, P.L.
Format: Journal Article
Language:English
Published: Elsevier Inc 01-12-1995
Online Access:Get full text
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Summary:The antitumor natural product RA-VII has been evaluated as an inhibitor of protein synthesis in vitro. Complete inhibition of protein synthesis in rabbit reticulocyte lysates is observed with 5 μM RA-VII. Mechanistic studies using purified elongation factors and ribosomes identify RA-VII as a peptidyltransferase inhibitor. Thus, similar to the related natural products bouvardin and RA-XII, RA-VII appears to function by binding to eukaryotic ribosomes.
ISSN:0045-2068
1090-2120
DOI:10.1006/bioo.1995.1032