Application of Immobilized Laccase on Polyurethane Foam for Ex-Situ Polycyclic Aromatic Hydrocarbons Bioremediation

• Published in: Journal of polymers and the environment Vol. 29; no. 7; pp. 2200 - 2213
• Main Authors: Perini, Brayam Luiz Batista, Daronch, Naionara Ariete, Bitencourt, Rodrigo Luiz, dos Santos Schneider, Andréa Lima, de Andrade, Cristiano José, de Oliveira, Débora
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-07-2021; Springer Nature B.V
• Subjects: Anthracene; Aromatic compounds; Benzo(a)pyrene; Biodegradation; Bioreactors; Bioremediation; Chemistry; Chemistry and Materials Science; Chrysene; Design of experiments; Diethyl phthalate; Environmental Chemistry; Environmental Engineering/Biotechnology; Environmental protection; Experimental design; Groundwater; Immobilization; Industrial Chemistry/Chemical Engineering; Laccase; Materials Science; Methylation; Original Paper; Oxidation; Phthalates; Pollutants; Polycyclic aromatic hydrocarbons; Polymer Sciences; Polyurethane; Polyurethane foam; Pyrene; Quinones; Tetradecane; PAHs; Enzymatic biodegradation; Bioremediation; Immobilized laccase; Polyurethane foam
• ISSN: 1566-2543; 1572-8919
• DOI: 10.1007/s10924-020-02035-9

There is growing concern about developing treatment technologies for the hazardous Polycyclic Aromatic Hydrocarbons (PAHs), because the rising levels of these compounds in the environment by human activities. The application of laccases has been evaluated as one of the most promising treatments. Thus, laccase immobilization on polyurethane foam (PUF)—low-cost material—was evaluated for bioremediation in batch mode of simulated groundwater using a combination of 16 polycyclic aromatic hydrocarbons as model pollutants. Conditions closer to a real contaminated site (non-optimal) were considered on our experimental design, leading to the formation of new degradation intermediaries, even more degraded than the usual ones. The bioremediation of PAH (mg L
−1
) using immobilized laccase on PUF reached 92.35% of removal for anthracene (Ant) and 97% for benzo(a)pyrene (BaP). After the treatment, the biodegradation products were identified as diisooctyl phthalate and tetradecane. The biodegradation mechanism was proposed, where PAHs oxidation processes and aromatic ring fission led to quinone and diethyl phthalate formation. Then, through the latter processes besides, polymerization and methylation, lead to the identified biodegradation product formation. The immobilized enzyme improvement in the removal yield of 8 of the other 14 PAHs tested in μg L
−1
, compared to the free counterpart. Laccase immobilized on PUF achieved final anthracene concentration of 0.95 mg L
−1
, up to 38 μg L
−1
of chrysene (77% removal) and 98 μg L
−1
of pyrene (32% removal), under the intervention limits of environmental protection policies. Thus, laccase immobilized on PUF for use in bioreactors can be considered a potential approach for PAHs bioremediation for an ex-situ treatment.