The ability of two indigenous bacteria isolated from Moroccan olive tree to promote the growth of olive seedlings in the presence of the pathogen Verticillium dahliae

• Published in: Biocontrol science and technology Vol. 33; no. 10; pp. 963 - 984
• Main Authors: Sallami, Abdessamad, Rabeh, Karim, Idrissi Lahsini, Abdelali, El Khedri, Hanane, Douira, Allal, El Modafar, Cherkaoui, Medraoui, Leila, Filali-Maltouf, Abdelkarim
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 03-10-2023; Taylor & Francis Ltd
• Subjects: Abiotic stress; Arid climates; Bacteria; Biofilms; Biological control; Exopolysaccharides; Fruit trees; Gram-negative bacteria; Mica; Olive; Pathogens; PGPR; Phosphate solubilisation; Plant growth; Potassium; Rhizosphere; Saline soils; Seedlings; Sodium chloride; Soil stresses; Strains (organisms); Trees; Verticillium dahliae
• ISSN: 0958-3157; 1360-0478
• DOI: 10.1080/09583157.2023.2263185

The rhizosphere of olive trees (Olea europaea L.) is a source of bacteria with a high potential for biocontrol and plant growth promotion. In the present study, two native olive tree gram-negative bacteria were isolated from the rhizosphere of century-old trees in the region of Zouala (south-eastern Morocco), known for its arid climate and saline soils. The strains Pantoea agglomerans and Enterobacter ludwigii exhibited high tolerance to high PEG and NaCl concentrations, and significant plant growth-promoting (PGP) potential in vitro. The two strains efficiently solubilised phosphate in its two forms (TCP and RP), and Mica as a source of potassium and produced IAA. The two strains exhibited in vitro inhibition ability against Verticillium dahliae with partial inhibition of the growth of the mycelium by the strain P. agglomerans MRC_ZO_17. Greenhouse experiments showed that olive tree seedlings treated with the E. ludwigii MRC_ZO_97 strain significantly improved growth (dry weight) in the presence and absence of the pathogen compared to untreated plants, while the P. agglomerans MRC_ZO_17 strain showed significant effects on seedling growth only in the presence of the pathogen V. dahliae OV1 defoliating pathotype. Both strains showed high exopolysaccharides (EPS) production and good biofilm formation, making them excellent plant growth-promoting rhizobacteria (PGPRs) and Biological Control Agents (BCAs) with great potential to survive in soil under stress conditions.