Influence of standing estrus before an injection of GnRH during a beef cattle fixed-time AI protocol on LH release, subsequent concentrations of progesterone, and steriodogenic enzyme expression

• Published in: Domestic animal endocrinology Vol. 42; no. 1; pp. 11 - 19
• Main Authors: Fields, S.D., Gebhart, K.L., Perry, B.L., Gonda, M.G., Wright, C.L., Bott, R.C., Perry, G.A.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 2012
• Subjects: 3-Hydroxysteroid Dehydrogenases - blood; 3-Hydroxysteroid Dehydrogenases - genetics; Animals; Cattle - physiology; Cholesterol Side-Chain Cleavage Enzyme - blood; Cholesterol Side-Chain Cleavage Enzyme - genetics; Cluster Analysis; Corpus Luteum - drug effects; Corpus Luteum - physiology; Corpus luteum function; Estrus - physiology; Female; GnRH; Gonadotropin-Releasing Hormone - pharmacology; Insemination, Artificial - methods; Insemination, Artificial - veterinary; LH pulses; Luteinizing Hormone - blood; Luteinizing Hormone - secretion; Male; Ovarian Follicle - diagnostic imaging; Ovarian Follicle - drug effects; Ovarian Follicle - metabolism; Ovarian Follicle - physiology; Pregnancy; Progesterone; Progesterone - blood; Real-Time Polymerase Chain Reaction - veterinary; Receptors, LH - blood; Receptors, LH - genetics; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA - chemistry; RNA - genetics; Ultrasonography; LH pulses; Progesterone; GnRH; Corpus luteum function
• ISSN: 0739-7240; 1879-0054
• DOI: 10.1016/j.domaniend.2011.08.002

Beef cows that exhibit estrus before fixed-time AI have been reported to have increased pregnancy success and increased concentrations of progesterone during the subsequent estrous cycle. Therefore, these experiments were conducted to evaluate if initiation of standing estrus before an injection of GnRH during a fixed-time AI protocol affected LH pulses, subsequent concentrations of progesterone, and luteal steroidogenic enzyme expression. In Experiments 1 and 2, cows were treated with the CO-Synch protocol (100 μg GnRH day −9, 25 mg PGF
2α day −2, and 100 μg GnRH day 0) and allotted to one of two treatments: 1) cows that initiated estrus before GnRH on day 0 (estrus; n = 5) or 2) cows that did not initiate estrus and were induced to ovulate by the GnRH on day 0 (no estrus; n = 5). In Experiment 1, blood samples were collected at 15-min intervals from 0 to 6 (bleed 1), 12 to 20 (bleed 2), 26 to 34 (bleed 3), and 40 to 48 (bleed 4) h after GnRH. Daily blood samples were collected for 17 d. Initiation of estrus before the GnRH injection had no effect on LH release or the pattern of progesterone increase; however, cows detected in estrus had overall increased (
P = 0.002) concentrations of progesterone compared with cows not in estrus. In Experiment 2, estrus was detected with the HeatWatch system. Location and size of the ovulatory follicle was determined on day 0 by transrectal ultrasonography at time of injection with GnRH. Blood samples were collected on days 3, 4, 5, 7, and 9; luteal tissue was collected on day 10 (n = 4 estrus and n = 9 no estrus) from corpus luteum (CL) originating from similar-sized follicles (13.0 to 16.0 mm). Total cellular RNA was extracted, and relative mRNA levels were determined by real-time reverse transcription PCR and corrected for GAPDH. There was no effect of estrus on CL weight or concentrations of progesterone. In addition, there was no effect of estrus, follicle size, or CL weight on luteal expression of LH receptor, StAR, CYP11A1, or 3βHSD. However, there was a correlation between follicle size and CL weight (
P = 0.01;
R
2 = 0.43); for every increase of 1 mm in follicle size, CL weight increased by 1.5 g. In summary, estrus did not influence release of LH, CL weight, progesterone concentrations, or expression of steriodogenic enzymes. However, as follicle size increased, CL weight increased; therefore, both follicle size and CL weight were associated with progesterone concentrations.