Ischemia deteriorates the spike encoding of rat cerebellar Purkinje cells by raising intracellular Ca2

• Published in: Biochemical and biophysical research communications Vol. 366; no. 2; pp. 401 - 407
• Main Authors: Zhao, Shidi, Chen, Na, Yang, Zhilai, Huang, Li, Zhu, Yan, Guan, Sudong, Chen, Qianfen, Wang, Jin-Hui
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 08-02-2008
• Subjects: Action Potentials; Animals; Brain Ischemia - physiopathology; Calcium - metabolism; Calcium Signaling; Cells, Cultured; Excitability; Intracellular Ca2; Ischemia; Purkinje Cells; Rats; Rats, Sprague-Dawley; Two-photon imaging; Whole-cell recording; Excitability; Whole-cell recording; Ischemia; Purkinje cells; Intracellular Ca2; Two-photon imaging
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2007.11.173

Ischemia-induced excitotoxicity at cerebellar Purkinje cells is presumably due to a persistent glutamate action. To the fact that they are more vulnerable to ischemia than other glutamate-innervated neurons, we studied whether additional mechanisms are present and whether cytoplasm Ca2+ plays a key role in their ischemic excitotoxicity. Ischemic changes in the excitability of Purkinje cells were measured by whole-cell recording in cerebellar slices of rats with less glutamate action. The role of cytoplasm Ca2+ was examined by two-photon cellular imaging and BAPTA infusion in Purkinje cells. Lowering perfusion rate to cerebellar slices deteriorated spike timing and raised spike capacity of Purkinje cells. These changes were associated with the reduction of spike refractory periods and threshold potentials, as well as the loss of their control to spike encoding. Ischemia-induced functional deterioration at Purkinje neurons was accompanied by cytoplasm Ca2+ rise and prevented by BAPTA infusion. Therefore, the ischemia destabilizes the spike encoding of Purkinje cells via raising cytoplasm Ca2+ without a need for glutamate, which subsequently causes their excitotoxic death.