Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087T Under Salt Stress

• Published in: Frontiers in microbiology Vol. 9; p. 618
• Main Authors: Han, Jian, Gao, Quan-Xiu, Zhang, Yong-Guang, Li, Li, Mohamad, Osama A. A., Rao, Manik Prabhu Narsing, Xiao, Min, Hozzein, Wael N., Alkhalifah, Dalal H. M., Tao, Yong, Li, Wen-Jun
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 29-03-2018
• Subjects: ectoine; halotolerant; hydroxyectoine; Microbiology; Nocardiopsis gilva; transcriptomic analysis; whole-cell catalysis
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2018.00618

The genus
Nocardiopsis
is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by
Nocardiopsis gilva
YIM 90087
T
under salt stress. The colony color of
N. gilva
YIM 90087
T
changed from yellow to white under salt stress conditions. Accumulation of ectoine and hydroxyectoine in cells was an efficient way to regulate osmotic pressure. The ectoine synthesis was studied by transferring the related genes (
ectA, ectB
, and
ectC
) to
Escherichia coli
. Transcriptomic analysis showed that the pathways of ABC transporters (ko02010) and glycine, serine, and threonine metabolism (ko00260) played a vital role under salt stress environment. The
ectABC
from
N. gilva
YIM 90087
T
was activated under the salt stress. Addition of exogenous ectoine and hydroxyectoine were helpful to protect
N. gilva
YIM 90087
T
from salt stress.