DESIGN: a guide to protein crystallization experiments

DESIGN: a guide to protein crystallization experiments

Existing guidelines for crystallizing proteins generally require substantial amounts of protein and scarce laboratory resources. To address this problem we have developed the program DESIGN, which, suited to daily laboratory usage, utilizes statistics when screening variables of crystallization. The...

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Bibliographic Details
Published in:Archives of biochemistry and biophysics Vol. 308; no. 2; p. 342
Main Author: Sedzik, J
Format: Journal Article
Language:English
Published: United States 01-02-1994
Subjects:
Calmodulin - chemistry
Cations, Divalent
Concanavalin A - chemistry
Crystallization
Crystallography, X-Ray
Indicators and Reagents
Lanthanum
Muramidase - chemistry
Myelin Proteins - chemistry
Proteins - chemistry
Software
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Description
Summary:Existing guidelines for crystallizing proteins generally require substantial amounts of protein and scarce laboratory resources. To address this problem we have developed the program DESIGN, which, suited to daily laboratory usage, utilizes statistics when screening variables of crystallization. The program DESIGN is described in considerable detail and examples of its outcome and capabilities are shown. In addition, a new scoring scale for crystallization trials is described. It is expected that the use of such an approach may provide additional constraints toward crystallizing the protein, even if the conditions applied are not successful.
ISSN:0003-9861
DOI:10.1006/abbi.1994.1049