Pituitary adenylate cyclase-activating polypeptide receptors of types I and II and glucagon-like peptide-I receptors are expressed in the rat medullary carcinoma of the thyroid cell line 6/23

• Published in: Endocrinology (Philadelphia) Vol. 135; no. 4; p. 1537
• Main Authors: Vertongen, P, Ciccarelli, E, Woussen-Colle, M C, De Neef, P, Robberecht, P, Cauvin, A
• Format: Journal Article
• Language: English
• Published: United States 01-10-1994
• Subjects: Adenylyl Cyclases - analysis; Animals; Base Sequence; Carcinoma, Medullary - chemistry; Carcinoma, Medullary - genetics; Carcinoma, Medullary - pathology; DNA, Neoplasm - analysis; DNA, Neoplasm - genetics; Gene Expression Regulation, Neoplastic; Glucagon-Like Peptide-1 Receptor; Iodine Radioisotopes; Molecular Sequence Data; Polymerase Chain Reaction - methods; Rats; Receptors, Cell Surface - analysis; Receptors, Cell Surface - genetics; Receptors, Cell Surface - physiology; Receptors, Glucagon; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide; Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I; Receptors, Pituitary Hormone - analysis; Receptors, Pituitary Hormone - genetics; Receptors, Pituitary Hormone - physiology; Receptors, Vasoactive Intestinal Peptide - analysis; Receptors, Vasoactive Intestinal Peptide - genetics; Receptors, Vasoactive Intestinal Peptide - physiology; RNA, Messenger - analysis; RNA, Messenger - genetics; Thyroid Neoplasms - chemistry; Thyroid Neoplasms - genetics; Thyroid Neoplasms - pathology; Tumor Cells, Cultured
• ISSN: 0013-7227
• DOI: 10.1210/endo.135.4.7925114

The expression of the messenger RNAs coding for glucagon-like peptide-I (GLP-I) receptor, VIP receptor, and pituitary adenylate cyclase-activating polypeptide (PACAP) receptor as well as the expression of the receptor proteins were demonstrated in the rat medullary carcinoma of thyroid cell line 6/23 by the following experiments: 1) RNA extraction, reverse transcriptase, and polymerase chain reaction with specific primers; 2) binding of the radiolabeled ligands [125I]GLP-I-(7-36)-NH2, [125I]PACAP-(1-27), and [125I]VIP and inhibition by, respectively, unlabeled GLP-I-(7-36)-NH2, PACAP-(1-27), and VIP; and 3) study of adenylate cyclase activation by the peptides and selective inhibition of the VIP/PACAP response by the antagonist [D-Phe2]VIP. Besides the highly selective GLP-I receptor, PACAP receptors of types I and II were present on the cell line and coupled to adenylate cyclase. PACAP stimulated the adenylate cyclase through type I and II receptors, whereas VIP interacted with type II receptors only. Messenger RNA analysis indicated that at least three splice variants of the PACAP type I receptor may be expressed in 6/23 cells.