Pyrene Based Fluorescent Turn-on Chemosensor for Sequential Detection of Fe3+ and Fe2+ Ions and its Application in Live Cell Imaging

• Published in: Journal of fluorescence Vol. 29; no. 3; pp. 797 - 802
• Main Authors: Dhivya, Rajasekaran, Gomathi, Asaithambi, Viswanathamurthi, Periasamy
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-05-2019; Springer Nature B.V
• Subjects: Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Buffer solutions; Chemical sensors; Chemoreceptors; Emission analysis; Ferric ions; Ferrous ions; Fluorescence; Ions; Original Article; Selectivity; Schiff base; Fluorescence probe; Cell imaging; Iron ions
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-019-02392-2

A simple pyrene-based “turn-on” chemosensor bearing isonizide namely 3-(pyren-1-yl methylene) pentane-2,4-diyldene di(isonicotinohydrazide) (PMPD) was designed and synthesized for detecting Fe
3+
and Fe
2+
ions in HEPES buffer solution at pH 7.4 (DMSO: H
2
O (1:9 
v
/
v
)) medium. The probe shows conducive selectivity for Fe
2+
and Fe
3+
ions over the other competitive metal cations. The detection limit was found to be 1.67 μM for Fe
3+
and 2.02 μM for Fe
2+
. The recognition mechanism of PMPD towards Fe
3+
and Fe
2+
has been examined in detail by absorption, emission, and ESI-MS studies. Moreover, “turn-on” fluorescence behavior of the probe was used to track iron ions in living cells.