Assessment of oxidative status, detoxification capacity and immune responsiveness in honey bees with ageing

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 298; p. 111735
• Main Authors: Spremo, Jelena, Purać, Jelena, Čelić, Tatjana, Đorđievski, Srđana, Pihler, Ivan, Kojić, Danijela, Vukašinović, Elvira
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-12-2024
• Subjects: Antioxidants; Apis mellifera; Detoxificants; Immunity; Senescence; Vitellogenin; Apis mellifera; Antioxidants; Senescence; Detoxificants; Vitellogenin; Immunity
• ISSN: 1095-6433; 1531-4332; 1531-4332
• DOI: 10.1016/j.cbpa.2024.111735

The honey bee (Apis mellifera L.), as an eusocial insect species, is an important model organism in research focusing on ageing and longevity, due to prominent seasonal lifespan plasticity within the worker caste (summer and winter worker bees). In this study, we employed a screening approach to evaluate several molecular parameters, providing comprehensive insights into the antioxidative (superoxide dismutase and catalase activity, reduced glutathione and sulfhydryl group content, total antioxidative capacity), detoxifying (glutathione S-transferase and acetylcholinesterase activity), and immune (phenol oxidase and glucose oxidase activity) status, as well as vitellogenin content, in the summer and winter generation of honey bees, across ageing stages and in two body compartments: the whole abdomen and the head. Summer worker bees were collected weekly for six weeks, while winter bees were collected monthly for five months. The results of our study clearly indicate a reduced overall antioxidative capacity of older groups of worker bees from both generations, while the parameters of immune responsiveness mostly contributed to the separation between the two generations based on season rather than age categories. Detoxification ability appeared to be more susceptible to environmental factors. An age-dependent increase in vitellogenin content was recorded in the abdomen, but without seasonal differences. These findings provide an excellent starting point for further investigations into age-related changes, particularly within the context of honey bee sociality.
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•Overall antioxidative capacity is reduced with ageing in summer and winter bees.•Immune responsiveness of bees (proPOx, POx and GOx) showed seasonal variations.•Detoxification ability was more susceptible to environmental factors, not ageing.•Vitellogenin content increased in the abdomen with ageing, but without seasonal differences.