Purification, host range, serology, and partial sequencing of dendrobium mosaic potyvirus, a new member of the bean common mosaic virus subgroup

• Published in: Phytopathology Vol. 85; no. 5; pp. 542 - 546
• Main Authors: Hu, J.S. (University of Hawaii, Honolulu.), Ferreria, S, Wang, M, Borth, W.B, Mink, G, Jordan, R
• Format: Journal Article Conference Proceeding
• Language: English
• Published: St. Paul, MN American Phytopathological Society 01-05-1995
• Subjects: Biological and medical sciences; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; DENDROBIUM; Fundamental and applied biological sciences. Psychology; GENE; GENES; HAWAI; HAWAII; HOTE; HUESPEDES; Microbiology; PESO MOLECULAR; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; POIDS MOLECULAIRE; POTYVIRUS; POTYVIRUS DEL MOSAICO COMUN FRIJOL; POTYVIRUS MOSAIQUE COMMUN HARICOT; PROTEINAS; PROTEINE; PURIFICACION; PURIFICATION; SECUENCIA NUCLEICA; SEQUENCE NUCLEIQUE; Systematics; Systematics. Structure, properties and multiplication. Genetics; Virology; Hawaii; Polynesia; Oceania; Monocotyledones; Genome organization; Plant pathogen; Nucleotide sequence; Taxonomy; Transmission; Bean common mosaic virus; Epidemiology; Virus; Orchidaceae; Dicotyledones; Angiospermae; Spermatophyta; New species; Potyvirus; Solanaceae; Experimental plant; Capsid; Potyviridae; Aminoacid sequence
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/Phyto-85-542

Dendrobium mosaic potyvirus (DeMV) was successfully transmitted from honohono orchids (Dendrobium superbum Reichb. f.) to Nicotiana benthamiana. The coat protein of the virus purified from N. benthamiana had a molecular weight of 34 X 10(3) in sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis, and the viral RNA was approximately 9.5 kb in size in denatured agarose gel electrophoresis. With a polyclonal antiserum produced against purified DeMV particles, DeMV was found to be widespread in honohono orchids in Hawaii. Based on serological reactions in indirect enzyme-linked immunosorbent assay with monoclonal antibodies, DeMV is a potyvirus of the bean common mosaic virus (BCMV) subgroup. DeMV-specific cDNA clones were obtained and sequenced. Sequence comparison using the coat protein amino acid sequence and the 3' untranslated region sequence showed that DeMV shares 88 to 91% and 85 to 95% identity, respectively, with those sequences of BCMV subgroup members. Data from host range studies suggest that DeMV behaves similarly to other potyviruses in the BCMV subgroup such as azuki bean mosaic virus (AzMV) and blackeye cowpea mosaic virus (BICMV). Based on available information, DeMV is considered a member of the BCMV subgroup