Comparative kinetic study of D-glucose oxidation by ruthenium(III) compounds catalyzed by FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase

• Published in: Biochemistry (Moscow) Vol. 68; no. 4; pp. 407 - 415
• Main Authors: Ivanova, E V, Ershov, A Yu, Laurinavicius, V, Meskus, R, Ryabov, A D
• Format: Journal Article
• Language: English
• Published: United States Springer Nature B.V 01-04-2003
• Subjects: Catalysis; Dehydrogenase; Enzymes; Glucose; Glucose - chemistry; Glucose - metabolism; Glucose Dehydrogenases - chemistry; Glucose Dehydrogenases - metabolism; Glucose Oxidase - chemistry; Glucose Oxidase - metabolism; Hydrogen-Ion Concentration; Kinetics; Molecular Structure; Oxidation; Oxidation-Reduction - drug effects; Quinolones - chemistry; Quinones - chemistry; Ruthenium; Ruthenium - chemistry; Ruthenium - pharmacology; Stereoisomerism; Structure-Activity Relationship; Time Factors
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1023/A:1023647813089

The comparative kinetic study of two glucose oxidizing enzymes, FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase, is presented in the artificial electron transfer mediator system based on ruthenium(III) compounds. It is demonstrated that FAD-dependent glucose oxidase and PQQ-dependent glucose dehydrogenase follow Michaelis kinetics in the D-glucose/ruthenium(III) system. PQQ-dependent glucose dehydrogenase is more active than FAD-dependent glucose oxidase in the process of D-glucose oxidation by ruthenium(III) compounds, this being due to the different catalytic mechanisms of these enzymes.