Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis

A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.

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Bibliographic Details
Published in:	Chemical communications (Cambridge, England) no. 3; p. 274
Main Authors:	Jahn, Michael, Chen, Hongming, Müllegger, Johannes, Marles, Jennifer, Warren, R Antony J, Withers, Stephen G
Format:	Journal Article
Language:	English
Published:	England 07-02-2004
Subjects:	Circular Dichroism Enzyme Stability Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Hydrogen-Ion Concentration Molecular Structure Mutation - genetics Temperature Thioglycosides - biosynthesis Thioglycosides - chemistry
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Description
Summary:	A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.
ISSN:	1359-7345
DOI:	10.1039/b313155f