Hydrogen gas and the gut microbiota are potential biomarkers for the development of experimental colitis in mice

• Published in: Gut microbiome (Cambridge, England) Vol. 5; p. e3
• Main Authors: Fujiki, Yuta, Tanaka, Takahisa, Yakabe, Kyosuke, Seki, Natsumi, Akiyama, Masahiro, Uchida, Ken, Kim, Yun-Gi
• Format: Journal Article
• Language: English
• Published: England Cambridge University Press 2024
• Subjects: Animal models; biomarker; Biomarkers; Colitis; Dextran; Diagnosis; Digestive system; Endoscopy; experimental colitis; Gases; Gastrointestinal tract; gut microbiota; Hydrogen; hydrogen gas; Inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Intestinal microflora; Lipocalin; Metabolites; Microbiota; Microorganisms; Sensors; VOCs; Volatile organic compounds; Weight; hydrogen gas; experimental colitis; biomarker; gut microbiota; inflammatory bowel disease
• ISSN: 2632-2897; 2632-2897
• DOI: 10.1017/gmb.2023.17

Inflammatory bowel disease (IBD) is a chronic disease characterised by repeated relapses and remissions and a high recurrence rate even after symptom resolution. The primary method for IBD diagnosis is endoscopy; however, this method is expensive, invasive, and cumbersome to use serially. Therefore, more convenient and non-invasive methods for IBD diagnosis are needed. In this study, we aimed to identify biological gas markers for the development of gut inflammation. Using dextran sulphate sodium (DSS)-induced colitis mouse models, five biological gases were analysed to identify predictive markers for the development of gut inflammation. Additionally, the correlation between the changes in gas composition, gut microbiota, and inflammatory markers was assessed. The hydrogen (H
) level was found to be negatively correlated with the level of lipocalin-2 (LCN2), a gut inflammation biomarker, and weight loss due to DSS-induced colitis. Furthermore, gut microbes belonging to the Rikenellaceae and Akkermansiaceae families were positively correlated with LCN2 levels and weight loss, whereas Tannerellaceae abundance was negatively correlated with LCN2 level and weight loss and positively correlated with H
levels. This study provides new insights for IBD diagnosis; the H
levels in biological gases are a potential biomarker for intestinal inflammation, and specific gut microbes are associated with H
level changes.