Salivary Calprotectin Levels and Bone Mineral Density in Post-Menopausal Women With and Without Chronic Periodontitis - An Evaluative Study

• Published in: Indian journal of dental research Vol. 35; no. 2; pp. 140 - 144
• Main Authors: Pavithra, J, Ramesh, Amitha, Thomas, Biju, Kumari, N Suchetha, Sharmila, K. P
• Format: Journal Article
• Language: English
• Published: Mangalore Medknow Publications and Media Pvt. Ltd 01-04-2024; Medknow Publications & Media Pvt. Ltd; Wolters Kluwer Medknow Publications
• Subjects: Bone density; Bone mineral density; Bones; calprotectin; Density; Development and progression; Developmental stages; Diagnosis; Enzyme-linked immunosorbent assay; Gum disease; Menopause; Menstruation; Osteoporosis; panoramic mandibular index; Periodontal diseases; Periodontitis; Post-menopause; Postmenopausal women; Statistical analysis
• ISSN: 0970-9290; 1998-3603
• DOI: 10.4103/ijdr.ijdr_522_21

Abstract Introduction: Menopause is a normal developmental stage in a woman’s life marking the permanent cessation of menstruation. Periodontitis and osteoporosis are noteworthy health concerns in post-menopausal women. Calprotectin is an inflammatory biomarker for precise diagnosis of periodontal disease. Materials and Methods: This study was performed to evaluate the variations in salivary calprotectin levels and bone mineral density in post-menopausal women with and without chronic periodontitis. The study included 24 post-menopausal women aged 45-57 years and was divided into group 1 (12 post-menopausal women without chronic periodontitis) and group 2 (12 post-menopausal women with chronic periodontitis). Unstimulated salivary samples, panoramic radiographs and clinical examination were performed. Results: The collected samples were combined with CALPRO antibody with horseradish peroxide (HRP) that results in the formation of antigen-antibody complex. Tetramethylbenzidine (TMB) solution was added that turned blue. Colour change was measured at 450nm. A human ELISA kit was used. Data collected were sent for statistical analysis. Discussion: There was a statistically significant difference in salivary calprotectin levels between groups 1 and 2 ( P = 0.016) and no statistical difference in mean bone mineral density levels between the two groups ( P = 0.235). A positive correlation was seen between salivary calprotectin levels and bone mineral density in group 2 ( P = 0.013).