Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150

Isolation and characteristics of highly active α-amylase from Bacillus subtilis-150

Partially purified enzyme preparation with specific activities of 153.7 U/mg for alpha -amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry of natural compounds Vol. 43; no. 4; pp. 454 - 457
Main Authors: Normurodova, K. T., Nurmatov, Sh. Kh, Alimova, B. Kh, Pulatova, O. M., Akhmedova, Z. R., Makhsumkhanov, A. A.
Format: Journal Article
Language:English
Published: 01-07-2007
Subjects:
Bacillus
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Partially purified enzyme preparation with specific activities of 153.7 U/mg for alpha -amylase and 0.15 U/mg for protease was produced by selective adsorption on starch. Enzymes were purified until homogeneous electrophoretically by gel-filtration over HW-55 TSK-gel with specific activities of 245 U/mg for alpha -amylase and 1.44 U/mg for protease. The optimum temperature and pH for purified alpha -amylase activity are 40-50 degree C and pH 6.0. The effects of various metal ions on the activity and stability of the enzyme were studied.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-3130
1573-8388
DOI:10.1007/s10600-007-0159-1