Molecular markers and GGE biplot analysis for selecting higher‐yield and drought‐tolerant maize hybrids

• Published in: Agronomy journal Vol. 113; no. 5; pp. 3871 - 3885
• Main Authors: Sedhom, Sedhom A., El‐Badawy, Mahmoud El. M., Hosary, Ahmed A. A. El, Abd El‐Latif, Mahmoud S., Rady, Asmaa M. S., Moustafa, Mahmoud M. A., Mohamed, Shereen A., Badr, Omnia A. M., Abo‐Marzoka, Sayed A., Baiumy, Khaled A., El‐Nahas, Marwa M.
• Format: Journal Article
• Language: English
• Published: 01-09-2021
• ISSN: 0002-1962; 1435-0645
• DOI: 10.1002/agj2.20778

Improving maize (Zea mays L.) genotypes for higher productivity and tolerance to drought stress depends mainly on physiological and molecular markers. Therefore, this study aims at breeding maize for drought tolerance and high potentiality by selection based on molecular markers, photosynthetic parameters; and easy graphic methods that help in selecting elite genotypes across diverse environments. An 8 × 8 half diallel analysis was used at two locations involving drought and normal irrigation treatments to study parental genetic diversity (GD) and combining ability (general combing ability [GCA] and specific combining ability [SCA]) in F1 of maize. Fingerprinting of parents was made using simple sequence repeat (SSR) markers. Fifty‐eight alleles were ranged from two to five alleles per locus with an average of 0.63 alleles per locus. The average of polymorphic information content (PIC) was 0.63. Cuvette temperature (oc) was lowest by the cross L14 × L36. The cross L8 × L34 expresses the highest value for Quantum sensor (μmol m–2 s–1), net CO2 assimilation rate and chlorophyll content. As for leaf diffusive resistance (LDR) four crosses exhibited significant desirable LDR values. Concerning rate of leaf transpiration (LTR) (μg cm−2 S−1) the cross (L5 × L104) gave the lowest value. Most hybrids exhibited desirable values for drought susceptibility index. For grain yield plant–1, five F1 crosses, that is, L5 × L34, L8 × L14, L8 × L14, L30 × L104, and L36 × L104 expressed the most desirable SCA effects. These crosses are promising in maize breeding programs. Based on GGE biplot analysis, genotype nos. 8 and 10 exhibited the highest grain yield plant−1 and ranked the first across all environments.
Core Ideas
Five new maize hybrids with higher yield potentiality and tolerance to drought were developed.
Crucial metabolic functions related to yield productivity of maize were identified.
The polymorphic information content and gene diversity of 40 SSR markers associated with drought tolerance were investigated.