Graphene 2D platform is safe and cytocompatibile for HaCaT cells growing under static and dynamic conditions

• Published in: Nanotoxicology Vol. 16; no. 5; pp. 610 - 628
• Main Authors: Lasocka, Iwona, Jastrzębska, Elzbieta, Zuchowska, Agnieszka, Skibniewska, Ewa, Skibniewski, M., Szulc-Dąbrowska, Lidia, Pasternak, Iwona, Sitek, Jakub, Hubalek Kalbacova, Marie
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-06-2022; Taylor & Francis Ltd
• Subjects: Animals; Anomalies; Cell Movement; Cell Proliferation; Cell Survival; Cell viability; Cultivation; Cytokinesis; Cytoskeleton; Glass substrates; Graphene; Graphite - toxicity; HaCaT Cells; Humans; Keratinocytes; Keratinocytes - metabolism; Lab-on-a-chip; Microfluidics; Mitochondria; Monolayers; Skin tests; Substrates; Toxicity testing; keratinocytes; lab on a chip; Graphene; cytoskeleton; mitochondria
• ISSN: 1743-5390; 1743-5404
• DOI: 10.1080/17435390.2022.2127128

The study concerns the influence of graphene monolayer, as a 2 D platform, on cell viability, cytoskeleton, adhesions sites andmorphology of mitochondria of keratinocytes (HaCaT) under static conditions. Based on quantitative and immunofluorescent analysis, it could be stated that graphene substrate does not cause any damage to membrane or disruption of other monitored parameters. Spindle poles and cytokinesis bridges indicating proliferation of cells on this graphene substrate were detected. Moreover, the keratinocyte migration rate on the graphene substrate was comparable to control glass substrate when the created wound was completely closed after 38 hours. HaCaT morphology and viability were also assessed under dynamic conditions (lab on a chip - micro scale). For this purpose, microfluidic graphene system was designed and constructed. No differences as well as no anomalies were observed during cultivation of these cells on the graphene or glass substrates in relation to cultivation conditions: static (macro scale) and dynamic (micro scale). Only natural percentage of dead cells was determined using different methods, which proved that the graphene as the 2 D platform is cytocompatible with keratinocytes. The obtained results encourage the use of the designed lab on a chip system in toxicity testing of graphene also on other cells and further research on the use of graphene monolayers to produce bio-bandages for skin wounds in animal tests.