Staphylococcus aureus cowan I and Branhamella catarrhalis as B lymphocyte mitogens. Culture conditions for optimal DNA synthesis and selective stimulation of human B lymphocytes

• Published in: Journal of immunological methods Vol. 51; no. 3; p. 279
• Main Author: Banck, G
• Format: Journal Article
• Language: English
• Published: Netherlands 1982
• Subjects: Animals; B-Lymphocytes - cytology; B-Lymphocytes - immunology; Cattle; Cells, Cultured; Culture Media; DNA - biosynthesis; Freezing; Humans; Immunoglobulin G - biosynthesis; Immunoglobulin M - biosynthesis; Lymphocyte Activation; Male; Middle Aged; Mitogens - pharmacology; Neisseria - immunology; Rosette Formation; Serum Albumin - pharmacology; Staphylococcus aureus - immunology; Thymidine - metabolism; Transferrin - pharmacology
• ISSN: 0022-1759
• DOI: 10.1016/0022-1759(82)90394-5

Staphylococcus aureus Cowan I and Branhamella catarrhalis that selectively stimulate human B lymphocytes to increased DNA synthesis in serum-free culture media were shown to do so also in 3 day cultures in medium containing human serum or albumin. After this time the transformed cells expressed little or no surface-bound immunoglobulin and did not form rosettes with sheep erythrocytes. When lymphocytes were cultured with bacterial mitogens in the presence of serum or albumin for more than 3 days transformed T cells appeared. The bacteria induced antibody production mainly of IgM class in lymphocytes cultured in albumin supplemented medium.