The BAS chromatin remodeler determines brassinosteroid-induced transcriptional activation and plant growth in Arabidopsis

• Published in: Developmental cell Vol. 59; no. 7; p. 924
• Main Authors: Zhu, Tao, Wei, Chuangqi, Yu, Yaoguang, Zhang, Zhenzhen, Zhu, Jiameng, Liang, Zhenwei, Song, Xin, Fu, Wei, Cui, Yuhai, Wang, Zhi-Yong, Li, Chenlong
• Format: Journal Article
• Language: English
• Published: United States 08-04-2024
• Subjects: Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Brassinosteroids - metabolism; Chromatin - genetics; Chromatin - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Gene Expression Regulation, Plant; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Transcriptional Activation; Brassinosteroid; growth and development; BAS-chromatin-remodeling complexes; SWI/SNF; chromatin accessibility; BZR1; phytohormone
• ISSN: 1878-1551
• DOI: 10.1016/j.devcel.2024.01.021

Brassinosteroid (BR) signaling leads to the nuclear accumulation of the BRASSINAZOLE-RESISTANT 1 (BZR1) transcription factor, which plays dual roles in activating or repressing the expression of thousands of genes. BZR1 represses gene expression by recruiting histone deacetylases, but how it activates transcription of BR-induced genes remains unclear. Here, we show that BR reshapes the genome-wide chromatin accessibility landscape, increasing the accessibility of BR-induced genes and reducing the accessibility of BR-repressed genes in Arabidopsis. BZR1 physically interacts with the BRAHMA-associated SWI/SNF (BAS)-chromatin-remodeling complex on the genome and selectively recruits the BAS complex to BR-activated genes. Depletion of BAS abrogates the capacities of BZR1 to increase chromatin accessibility, activate gene expression, and promote cell elongation without affecting BZR1's ability to reduce chromatin accessibility and expression of BR-repressed genes. Together, these data identify that BZR1 recruits the BAS complex to open chromatin and to mediate BR-induced transcriptional activation of growth-promoting genes.