Rat pancreatic islet cells in primary culture: occurrence of giant cells amenable to patch clamping

Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40-50 micron) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amena...

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Bibliographic Details
Published in:	Experientia Vol. 41; no. 1; pp. 116 - 118
Main Authors:	Mathers, D A, Buchan, A M, Brown, J C, Otte, S C, Sikora, L K
Format:	Journal Article
Language:	English
Published:	Switzerland 01-01-1985
Subjects:	Animals Cell Membrane - physiology Cells, Cultured Electrophysiology Insulin - metabolism Ion Channels - physiology Islets of Langerhans - cytology Islets of Langerhans - physiology Male Rats Rats, Inbred Strains
Online Access:	Get full text
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Summary:	Neonatal and adult rat pancreatic islet cells were maintained in dissociated cell culture for up to three weeks. The unexpected occurrence of giant (40-50 micron) cells was noted, some of which reacted positively to an insulin antiserum, indicating the presence of insulin. The giant cells were amenable to study using the extracellular patch clamp technique, which was used to demonstrate a population of membrane channels gating outwardly directed current in these cells.
ISSN:	0014-4754 1420-9071
DOI:	10.1007/BF02005906