An efficient Agrobacterium tumefaciens-mediated transformation system of Taraxacum kok-saghyz Rodin (Russian dandelion)

• Published in: In vitro cellular & developmental biology. Plant Vol. 59; no. 3; pp. 413 - 423
• Main Authors: Peng, Cunzhi, Chang, Lili, Wang, Dan, Xie, Quanliang, Zheng, Xingmei, Xu, Bingqiang, Tong, Zheng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-06-2023; Springer Nature B.V
• Subjects: Agrobacterium tumefaciens; Benzyladenine; Biomedical and Life Sciences; Biosynthesis; Cell Biology; Developmental Biology; Efficiency; Elongation; Explants; Genes; Genetic transformation; Genotype & phenotype; Genotypes; Germplasm; Hygromycin; Latex; Leaves; Life cycles; Life Sciences; Naphthaleneacetic acid; Natural rubber; Plant Breeding/Biotechnology; Plant Genetics and Genomics; Plant introductions; Plant Sciences; Plant Tissue Culture; Rubber; Seedlings; Seeds; Shoots; Taraxacum; Technical services; Tissue culture; Transformations; Beijing China; China; Genetic transformation system; Rodin; Rubber elongation factor; Rubber-yielding plant
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1007/s11627-023-10352-6

Taraxacum kok-saghyz
Rodin (TK) is an excellent alternative to
Hevea brasiliensis
because of its high yield of latex, wide growth range, and short life cycle, and it is also an ideal plant for natural rubber research through genetic transformation. This study aimed to report an efficient
Agrobacterium tumefaciens
-mediated transformation system of TK using leaves as explants. A TK genotype, named 12–16, that is suitable for tissue culture was obtained from a wild germplasm repository in Xinjiang. The multiplication rate of TK genotype 12–16 reached 3.72 adventitious shoots per explant through cultivation on the shoot induction medium of Murashige-Skoog (MS) nutrient medium supplemented with 1.5 mg L
−1
6-benzylaminopurine and 0.1 mg L
−1
naphthaleneacetic acid. Moreover, the average length of adventitious shoots was 3.07 ± 0.78 cm after 60 d in culture. Based on the transformation system, two genes of rubber elongation factor 138 (
REF138
) and rubber elongation factor 258 (
REF258
), driven under an enhanced 35S promoter, were transformed into the genotype 12–16 through
Agrobacterium tumefaciens
GV3101 mediated genetic transformation. The transformation process from leaf explants to hygromycin-resistant seedlings lasted approximately 3 to 4 mo, and the transformation efficiency reached 23.12 to 24.05%. This study established a highly efficient transformation system based on the TK genotype 12–16 and provided an important technical support for rubber biosynthesis research.